Echo cloning system:recent advance of DNA recombination technology;
镜像克隆系统:DNA重组技术的新进展
This study is focused on Corynebacterium glutamicum, an important producer in amino acids industry,by the protocol with construction of the in frame deletion fragments of targeting genes,application of DNA recombination technique and so on.
通过目标基因内部缺失片段的获得以及DNA重组交换等技术的有效运用 ,在氨基酸工业重要生产菌谷氨酸棒杆菌中 ,成功地定点构建了两个目标研究基因的单基因缺失菌株和双基因缺失菌株 ,并通过了PCR和测序等方法的分析验证。
The reaction conditions for DNA recombination are optimized The cohesive_blunt end ligation is going on by two step And this raises the efficiency of DNA recombination greatl
优化DNA重组的反应条件 ,将粘端一平端连接分两步进行 ,大大提高了DNA重组的效
Furthermore,the application of recombinant DNA techniques in the genetic manipulation of S.
本文简要综述了重组DNA技术应用于酿酒酵母遗传操作的研究进展,并指出了重组DNA技术作为新的遗传操作的手段,其应用性明显优于经典的遗传操作方法。
Genoraic DNA library is a collection of recombinant DNA clones which contain the complete gene s random fragments.
基因组DNA文库(genomic DNA library)是含有某种生物体全部基因的随机片段的重组DNA克隆群体。
Therapeutic Effects of NGI Recombinant DNA Vaccine on Transgenic AD Mice
NGI重组DNA疫苗对转基因AD小鼠的治疗作用
Some success in interferon production by bacteria has recently been achieved by the recombinant DNA technique.
近年曾经应用重组DNA技术使细菌产生干扰素获得了一定成效。
The Study of Inhibitory Effect on HPV Induced by Recombinant DNA Vaccine CRT-HPV and Gene Silencing by Specific siRNA;
CRT-HPV重组DNA疫苗和RNA干扰技术对HPV的抑制作用研究
Cloning and Expression of Recombinant Ag85A DNA;
重组Ag85A DNA的克隆与表达
the technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism.
从不同生物体中切分dna分子重新组合以在体外准备重组dna的技术。
Comparative Study on the Method for Isolation of the Recombinant Plasmid pRSET DNA;
重组质粒pRSET DNA提取方法的探究
V(D)J recombination and DNA non-homologous end joining
V(D)J重组与DNA非同源末端连接
Using Alkaline Lysis Method for Recombine Plasmid DNA Extraction and PCR Verification
碱裂解法提取重组质粒DNA及PCR验证
Comparison of fluorescence and DNA hybridization methods for the determination of residual DNA in recombinant products
荧光法和DNA杂交法检测重组技术产品中残余DNA的比较
paranemic joint
平行汇接[由双链DNA分子在重组区解旋而形成]
Detection of Exogenous Residual DAN in Recombinant Interleukin 2 Products
重组白细胞介素2制品外源残余DNA的检测
Recombinant DNA is also an excellent example of this problem.
重组体DNA也是这类问题的一个极好的例子。
Large-Scale Production and Characterization of Recombinant Plasmids;
重组质粒DNA的规模化生产工艺及质量鉴定研究
Cloning of P-Genome Specific Repetitive DNA Sequences from Agropyron Gaertn and Its Application;
冰草P基因组特异DNA重复序列的克隆及应用
Evaluation of the Effectiveness and Safety of the Thermo-Treatment Process to Dispose of Recombinant DNA Waste;
热处理废弃重组质粒DNA的有效性与安全性分析
Purification of Recombinant DNA Polymerase in E.coli;
重组大肠杆菌DNA聚合酶的提取及纯化研究
The environmental factors affecting the efficiency of thermo-treatment for disposal of waste plasmid DNA
废弃重组质粒DNA热处理效率的环境影响因素
Determination of residual host genomic DNA in recombinant products by real-time PCR
实时定量PCR检测重组技术产品中宿主基因组DNA残留