luciferase

1. The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine was determined by dual-luciferase reporter assay.: 通过双荧光素酶报告基因测定法测定了IGFBP-3对由三碘甲状腺素刺激的生长激素启动子活性的影响。

2. Methods The method of luciferase reporter gene was used.: 方法采用荧光素酶报告基因方法。

3. The species, structure and applications of luciferase were researched.: 文章就荧光素酶的种类、结构及应用进行了研究。

4. We addressed this question with the method of luciferase reporter gene.: 我们用荧光素酶报告基因的方法，探讨了这个问题。

5. Luciferase reporter gene technique plays an important role in high throughput screening.: 荧光素酶报告基因分析技术在药物的高通量筛选中起着重要的作用。

6. The cloning of luciferase from fireflies, which also took place in 1985, proved just as useful.: 同年，克隆荧火虫的荧光素酶也取得成功，这个非常有用。

7. We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.: 我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。

8. Results A series of truncated NOS1 promoter luciferase reporter vectors were constructed and identified.: 结果构建并鉴定了系列截短的NOS1 启动子萤光素酶报告基因载体；

9. The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase.: 相对荧光素酶活性法检测GR转录激活功能的变化。

10. Transcriptional activation activity of variant was evaluated through methods of relative activity of luciferase.: 相对荧光素酶法检测转录激活活性。

11. The mice were also regularly injected with luciferin, so that the luciferase would have the fuel it needed to glow.: 小鼠还定期注射荧光素，以便荧光素酶能够持续发光。

12. The investigators used genetically engineered E. coli cells to produce luciferase as a function of AHL concentration.: 研究者用基因工程的大肠埃希菌细胞生产荧光素酶作为AHL浓度函数。

13. Therefore, the luciferase has enhanced stability and long storage time, and can be used more conveniently and rapidly.: 虫荧光素酶稳定性加强，保存时间长，使用更为方便快捷。

14. Dual-luciferase reporter vector and mutated dual-luciferase reporter vector analysis validated that they are interacted.: 双荧光素酶报告载体和突变双荧光素酶报告载体分析证实了它们能够相互作用。

15. The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions.: 随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。

16. ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.: 目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体，并验证其转录活性。

17. All three mutants showed higher luciferase activity than that of the wild type, and the V85I mutant showed the highest activity.: 三个突变异种均显示比野生型具有更高的荧光素酶活性，并且V85I突变异种活性最强。

18. The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.: 探讨感光受体外周蛋白结合蛋白（PBP）在体外是否有促进变性蛋白质复性的作用。

19. In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.: 核净未能引起荧光素酶活性值的增加，但菌核净能抑制DHT依赖的AR活性，并呈现。

20. Objective The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.: 目的探讨感光受体外周蛋白结合蛋白（PBP）在体外是否有促进变性蛋白质复性的作用。

21. The transcriptional activation function of GR was evaluated through the detection of relative luciferase activity after dexamethasone treatment.: 相对荧光素酶活性法检测地塞米松作用后GR转录激活功能的变化。

22. The effect of MDM2 on the transcriptional activity of er was detected by the reporter gene containing estrogen responsive elements luciferase (ERE-LUC).: 以含雌激素反应元件的荧光素酶(ERE - LUC)为报告基因，通过检测荧光素酶活性来确定MDM2是否对ER有转录调节因子的作用。

23. Objective to study the DTA vector containing the Ewing family of tumors EWS-FLI1 binding sequence depresses the co-transferred luciferase reporter plasmid.: 目的研究含尤文家族肿瘤ews FLI1结合序列的DTA载体对报告基因表达的抑制作用。

24. METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.: 方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；

25. The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.: 瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。

26. ATP-Bioluminescence method allowed the rapid quantification of microorganisms by measuring their intracellular ATP in the firefly luciferin-luciferase BL reaction.: 生物发光法是一种用于分析待检测样品中有无微生物及其数量的方法。

27. Though they haven’t published their results yet, last year his team got a synthetic ribosome to self-assemble and produce luciferase, the protein that makes fireflies glow.: 去年，丘奇的研究团队通过自组装获得了合成核糖体，并产生了荧光素酶，这种蛋白质可以使萤火虫发光。

28. In HEK293T, Dual-Luciferase Reporter Assay System was used to evaluate the effect of E4B on TLR pathway, and co-immunoprecipitation was used to identify interactive protein.: 在HEK293T细胞中，通过双荧光报告系统检测E4B对TLR信号通路的影响，并且用免疫共沉淀的方法鉴定E4B的相互作用蛋白质。

29. The simplest light-producing reactions require an organic molecule, known as a luciferin, to be oxidised by oxygen, a process that is helped along by an enzyme known as luciferase.: 最简单的发光反应需要一种叫做荧光素有机分子，在荧光素酶的帮助下，进行被氧气氧化的氧化反应。

30. The simplest light-producing reactions require an organic molecule, known as a luciferin, to be oxidised by oxygen, a process that is helped along by an enzyme known as luciferase.: 最简单的发光反应需要一种叫做荧光素有机分子，在荧光素酶的帮助下，进行被氧气氧化的氧化反应。

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