Optimization of Fermentation Procedure for Recombinant E.coli Strain with D-GPEi Plasmid DNA;
重组质粒DNA D-GPEi工程菌发酵工艺的优化
Large-scale Production of Plasmid DNA;
质粒DNA的大规模制备
Experimental research on transformation of plasmid DNA into Escherichia coli XL1-Blue MRF′ strain mediated by electroporation.;
电穿孔法介导质粒DNA转化大肠杆菌XL1-Blue MRF′菌株的实验研究
Structural Changes of pUC18 DNA Irradiated by Ionizing Radiation Analysed by FT-Raman Spectroscopy;
电离辐射质粒pUC18 DNA结构变化的傅里叶变换拉曼光谱分析
Objective To construct a therapeutic mouse NGF DNA plasmid and optimize the procedure for pilot production of the plasmid.
目的构建小鼠NGF基因治疗型DNA质粒,并进行中试工艺的优化。
Objective To control the quality of mouse nerve growth factor (NGF) DNA plasmid for gene therapy.
目的对小鼠神经生长因子(NGF)基因治疗型DNA质粒进行质量控制。
Extraction of plasmid-like DNA and high-quality total DNA from Porphyra yezoensis;
条斑紫菜高纯度总DNA及其质粒状DNA的提取
A DNA algorithm for mining optimal ingredient pattern of traditional Chinese medicine is presented here, which is based on plasmid.
提出了一种用于中药配方优化的DNA算法,该算法基于质粒DNA技术。
An easy and feasible technology for plasmid purification was established with the optimized proportion between the quantity of CTAB and plasmid, combined with the specific solution for DNA release and TritonX-114 for endotoxin removal.
通过直接在大肠杆菌碱裂解上清中加入十六烷基三甲基溴化铵(CTAB),优化CTAB与质粒DNA量的比例、质粒DNA选择性释放溶液的选择和TritonX-114的使用,建立了简单、易行的大规模质粒DNA纯化工艺。
Moreover uses in the gene therapy and DNAvaccine plasmid DNA demand increases, the industri.
质粒DNA应用于核酸疫苗和基因治疗的需求快速增长,需要建立一套大规模生产质粒DNA工艺及与其相应的检测方法。