supernatant

1. PH0016 is a Paecilomyces lilacinus, the fermental supernatant has immunoenhancement activity, the active metabolites were worthy to be studied further.: PH0016是一株淡紫色拟青霉，其发酵上清具有一定的免疫增强活性，值得进一步研究其活性代谢产物。

2. Use only this supernatant in subsequent steps.: 只用这部分上清进行以下步骤。

3. Human CD3AK cells were transfected by viral supernatant.: 用病毒上清感染人CD3AK细胞。

4. The culture supernatant could stimulate the neurite outgrowth of PC12 cells.: 阳性克隆培养上清能促进PC 12细胞突起生长。

5. DHBV-DNA in the cytoplasm, cell nucleus and medium supernatant was assayed over time.: 分时段检测细胞核、细胞浆及培养上清的DHBV - DNA。

6. Human IL-4 biological activity was identified from the culture supernatant by FACS analysis.: 经FACS测定证实细胞的培养上清中具有人IL - 4的生物学活性。

7. The protein of procaryotic expression exists both in the supernatant fluid and the sediment.: 原核表达的蛋白质无论在沉淀中还是在上清中均有表达。

8. The hematopoietic cells were cocultivated with concentrated virus supernatant and interleukins.: 骨髓造血细胞与浓缩病毒上清及细胞因子组合共培养。

9. Objective: To study whether supernatant of C6 glioma cells can induce migration of neural stem cells in vitro.: 前言：目的：体外观察c6胶质瘤细胞的培养上清是否有诱导神经干细胞迁移的作用。

10. It is this very uneven, so that the supernatant Shenzhen drink team reduced to avoid relegation fight for the team Yunan.: 正是这种极不平衡的数据，令深圳上清饮队沦落到了为保级而战的鱼腩球队。

11. Antigen prepared from infected cells culture supernatant which was concentrated 100 times was positive to OvLV by AGID test.: 病毒培养液经过100倍浓缩，制成抗原进行琼扩检测呈阳性反应。

12. Supernatant from freeze thawing haemocytes was applied for the tests of immunoprecipitation, bacteriostasis, and phagocytosis.: 取冻融的血淋巴细胞上清进行免疫沉淀、抑菌、吞噬杀菌实验。

13. When alcohol density of fermentation supernatant was 50% and 70%, two kinds of water-solubility polysaccharide can be acquired.: 当发酵液中乙醇浓度为50%、70%时，分别得到两种水溶性很好的多糖。

14. Objective Investigate the relationship between cytokine level in MLC supernatant and acute rejection after renal transplantation.: 目的探讨混合淋巴细胞培养（MLC）中细胞因子水平与急性排斥反应的关系。

15. Conclusion: This result suggests the addition of ethanol should be optimized according to WCCE and CEA to obtain better supernatant.: 结论：为了取得更好的醇沉效果，生产中应该根据浓缩液含水量和待用乙醇的体积分数适当调整加醇量。

16. Objective: To investigate the effects of AMB (Astragalus Mongholicus Bge) on the immunosuppression of tumor cell line S180 supernatant.: 目的：研究黄芪对S180肿瘤培养上清免疫抑制作用的影响。

17. RESULTS: Fusion protein existed in supernatant of the bacteria lysate and its expression level was about 7% of the total bacteria protein.: 结果：融合蛋白以可溶性形式存在于细菌裂解液的上清中，其表达量为菌体总蛋白量的7%。

18. The extraction process of hogwash fat was simulated to produce hogwash fat by mixing edible oil and sewage and taking the supernatant oil.: 本文将不同浓度的污水与食用油搅拌混合，经静置后取其上层油层来人工模拟劣质泔水油。

19. The mechanical scrape wound model of mouse NIH3T3 cells was used to identify the action on promoting the wound healing of this supernatant.: 采用细胞机械创伤模型，检测此上清促进创伤愈合作用。

20. The promoting effect to Suillus luteus of the ethanol supernatant dried at room temperature is better than which dried at room temperature.: 室温条件下干燥得的乙醇沉淀物对菌丝生长的促进效果比高温条件干燥得的沉淀物好；

21. Conclusion: it is identified that the splenocytes culture supernatant has growth factor like action and can substitute for serum in the medium.: 结论：推测梅花鹿脾细胞培养上清具有生长因子样活性，并在细胞培养基中代替血清的作用。

22. The proliferation activity of fetal spleen lymphocytes stimulated with the culture supernatant of human fetal pituitary (FPS) was measured by MTT.: 采用MTT法测定人胎儿垂体培养上清(FPS)刺激胎脾淋巴细胞增殖反应。

23. The mechanical cell breaking could obviously increase the rate of autolysis and improve yield and the content of amino acid nitrogen in supernatant.: 机械破碎可以明显缩短自溶时间，提高酵母抽提物的氨基氮含量和得率。

24. ResultsThe supernatant of lymphocytes modified by BDNF gene decreased the growth inhibitory rate and apoptosis rate of PC12 cells induced by H2O2 and DA.: BDNF基因修饰的大鼠淋巴细胞的培养上清可降低H2O2和DA对PC 12细胞生长的抑制率并能抑制H2O2和DA诱导PC 12细胞凋亡。

25. The monocytes were co-incubated with supernatant from human marrow(mesenchymal) stem cells culture, and flow cytometer was used to detect surface antigen.: 与人骨髓间充质干细胞培养上清共孵育。流式细胞仪检测表面抗原。

26. Place tubes containing magnetic beads in the magnetic Separation Rack, wait 1 to 2 minutes for the solution to clear, and then carefully remove supernatant.: 将含有磁珠的管子置于磁性分离架中，等1 -2分钟至溶液澄清，然后小心移除上清。

27. The expression supernatant was purified with cation exchange chromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.: 表达上清经阳离子交换层析分离纯化为单一组份，SDS - PAGE显示相对分子质量为6 500。

28. The crude lipopeptide was isolated by acid precipition method from C-1 fermentation supernatant. The antibacterial activity of this lipopeptide was verified.: 通过酸沉淀法从发酵液上清中分离粗脂肽，并确定脂肽是发酵液上清中具有抑菌活性的物质。

29. Along with the addition of ammonia, the concentration of McAb in culture supernatant was decreased, however, the specific production rate of it was increased.: 氨的添加降低了培养上清中的单抗浓度，但提高了单抗的比生产速率。

30. Along with the addition of ammonia, the concentration of McAb in culture supernatant was decreased, however, the specific production rate of it was increased.: 氨的添加降低了培养上清中的单抗浓度，但提高了单抗的比生产速率。

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