The mono-layer gel plates were made by filling the polystyrene ponds with 1% LMA embedded lymphocytes and exposed to 6 Gy γ rays irradiation,then analysis were performed by comet assay in alkaline solution.
方法 自制聚苯乙烯孔槽的载胶片 ,将 1%低熔点凝胶与淋巴细胞混合后单层铺胶 ,经 6Gyγ射线照射后进行碱性单细胞凝胶电泳 ,观察胶面和淋巴细胞的DNA损伤情况。
The aim of this investigation is to study the effect of X ray irradiation on the viability of VSMC and the cellular mechanism, depending on which irradiation inhibits VSMC proliferation.
方法 体外培养VSMC ,给予单次 8MVX射线照射 ,源皮距为 10 0cm ,剂量率 40 0cGy/min。
Objective To investigate the effect of antisense heat shock protein (hsc70) on expression and stability of wild type p53 protein (wtp53) after X ray irradiation.
目的 探讨反义热休克蛋白 70 (hsc70 )在X射线照射下对野生型p5 3(wtp5 3)的影响。
0 Gy X-ray irradiation increased significantly when the mice were injected with PSH before irradiation,and the survival days were delayed,the protection index was 1.
进一步研究发现 ,小鼠经 8 0GyX射线照射后 72h ,黄蘑多糖能明显降低受照小鼠肝脏中LPO含量 (P <0 0 1) ,且显著增强其SOD、GSH -PX 和CAT活性 (P <0 0 5 )。
Methods(Damages of) liver cells in rats after γ-ray irradiation with a total dose of 8 Gy were investigated.
方法在体外利用γ射线照射诱导大鼠肝细胞损伤,在亚细胞水平上观察肝细胞的损伤修复情况与线粒体自噬的关系,并利用分子生物学技术进行DNA凋亡片段的分析和线粒体RNA的RT-PCR分析。