RT-PCR was conducted to detect the expression of TERT mRNA and immunocytochemistry assay was used to observe the nuclear translocation of NF-κB.
方法体外培养雌激素受体阳性(ER+)和阴性(ER-)乳腺癌细胞MCF-7和MDA-MB-231,MTT法检测细胞增殖的影响;RT-PCR法观察端粒酶逆转录酶TERT基因mRNA的表达;免疫细胞化学染色观察NF-κB p65的核移位。
Objective By studying the subcellular localization of Syk(L)in breast cancer cells and the mechanism of its nuclear translocation,explore the possible mechanism of Syk(L)tumor suppression function.
构建绿色荧光蛋白(GFP)和Syk(L)核移位相关功能域融合蛋白质粒,转染入Cos7细胞内,荧光显微镜下观察融合蛋白在细胞内的分布。
The geometries, vibrational frequencies, nucleus independent chemical shifts (NICS) and stabilities of the isomers were discussed.
采用量子化学HF ,B3LYP和MP2方法 ,选用 6 3 1G ,6 3 11G ,cc pVDZ和cc pVTZ基组 ,对C2 4团簇的 6种异构体进行了优化 ,并对它们的几何构型、振动频率、核独立化学位移 (NICS)和稳定性进行了讨论 ,比较C2 4团簇各种异构体的稳定性 。
The analysis of molecular orbital and nucleus independent chemical shift for these systems reveals that there is no connection between aromatic and bond localization,and the strength of diamagnetic ring current is governed by the substituent.
用Gaussian 03程序,采用密度泛函理论方法,对桥环三元环苯的结构和芳香性进行研究,优化得到了其稳定平衡构型,讨论了结构,通过核独立化学位移(NICS)和分子轨道(MO)分析,确定了桥环三元环苯的芳香性与键的定域之间是没有联系的,分子中的各个环的环电流大小是由取代基所决定的。
Topological Resonance Energy(TRE) and Percentage Topological Resonance Energy(%TRE) methods were applied to all the boron-or nitrogen-fullerene C20-2nX2n(X=B,N;n=1,2,3,4) isomers to investigate their aromaticity and compared with the Nucleus Independent Chemical Shift(NICS) values.
用拓扑共振能(TRE)和百分拓扑共振能(%TRE)方法对硼和氮掺杂富勒烯C20的芳香性进行了研究,并与核独立化学位移(NICS)方法的结果进行了比较。
The stabilities of the BmN (m=2-9) were analysized by means of energy difference, nucleus independent chemical shifts (NICS), and secondary energy differences of the ground state structures.
通过对基态结构的异构化能、核独立化学位移(NICS)和能量二次差分的讨论,得到了BmN(m=2-9)团簇结构的稳定性信息。
The geometries, natural bond orbital (NBO), electron structures, vibrational spectra, binding energies, nucleus independent chemical shifts (NICS) and energy secondary differences of ring and cage structures were discussed at the same level.
讨论了环状与笼状稳定团簇的几何构型、自然键轨道 (NBO)、振动频率、结合能、核独立化学位移 (NICS)和能量二次差分 ,得到了 (BN) n(n =1～ 16)团簇结构的稳定性信息 。
Nucleus-Independent Chemical Shifts (NICS) has been widely used in characterizing aromaticity and antiaromaticity of rings, clusters, transition states, transition metal complexes, etc, since it was proposed in 1996 by Schleyer et al.
1996年Schleyer等人提出了核独立化学位移(NICS)的概念,从此NICS被广泛用于判别环状化合物、团簇化合物、过渡态结构和过渡金属化合物的芳香性和反芳香性,他们指出作为探针的Bq原子应该放在分子中心和离环1 ?处。
Structural Basis for Translocation between Cytoplasm and Nucleus and Molecular Mechanism for Initiation Function of Cell Apoptosis of the BRD7 Protien;
BRD7蛋白胞浆/胞核移位的结构基础及细胞凋亡始动功能的分子机制
Involvement of Mitochondrio-nuclear Translocation of Apoptosis-inducing Factor in Dopaminergic Neurons of Substantia Nigra in Mouse Model of Parkinson's Disease
帕金森病小鼠黑质多巴胺能神经元凋亡诱导因子核移位的研究
displacement law of radionuclide
放射性核素位移定律
Potential Kernel of Drift Motion of Brown and its Martin Boundary;
漂移Brown运动的位势核与Martin边界
NMR Design and Spectral Shift Data Analysis
核磁共振测前设计及谱位移资料解释
Changes of nuclear mass and nuclear size cause isotope shifts.
原子核质量和大小的不同引起同位素移动。
An Algorithm for Finding a Nonzero Kernel of a Structured Matrix With Displacement Rank Approach
用位移秩方法求结构矩阵非零核空间的算法
Preliminary study on CXCR4 nuclear localization sequence in metastatic renal cell carcinoma
肾癌转移相关CXCR4基因核定位序列的初步研究
Choice of network location and performance for focus firm migration:a theoretical model
集群核心企业迁移的网络位置选择与迁移绩效:一个理论模型
Objectie. To quantify sagittal migration of the lumbar nucleus pulposus in 6 functional positions.
目的：定量测量在6种功能性体位情况下椎间盘髓核的矢状移位。
Transfer of a segment of DNA to a new position on the same or another chromosome, plasmid, or cell.
换位把脱氧核糖核酸的一个片断转移到同一个或另外的染色体、质体或细胞上的一个新位置上
The Perioperation Nursing of Hepatic Transplantation of Hepatic Bean Nucleus Denaturalization Back Type
肝豆状核变性背驮式原位肝移植术围手术期护理
Integration of NMR DTW Logs and DTE Logs to Improve the Oilbed Interpretation Accuracy.
综合利用核磁谱差分与谱位移测井提高油层解释精度
Natural Bond Orbital Analysis of Nucleus-Independent Chemical Shifts of Aromaticity and Antiarmaticity;
自然键轨道法分析芳香性和反芳香性的核独立化学位移
The Immunity of Multi-epitope DNA Vaccine Against HIV and the Construction of the Fowl-pox Viral Transfer Vector;
HIV多表位核酸疫苗免疫实验及鸡痘病毒转移载体的构建研究
Treatment of Human Hepatocellular Carcinoma in Nude Mice Orthotopic Implantation Model with IGF-IR Antisense Phosophorothioate Oligodeoxynuclieotides;
IGF-1R硫代反义寡核苷酸治疗人肝癌裸鼠原位移植瘤的研究
Research and Testing of the Super Distributed Home Location Register Based on the 3G Core Network;
基于3G核心网的超级分布式归属移位寄存器测试的研究
Research on Position Excursion of Core Enterprise Within Supply Chain;
核心企业在供应链中的位置及决定其漂移的4个维度