Aim: To evaluate the mucosal immune response, anti-fertility, change of ovarian histology induced by oral immunization with DNA contraceptive vaccine Chitosan/pVAX1-pZP3α nanoparticles and explore the feasibility of taking advantage of mucosal immunity to overcome ovary dysfunction which was often caused by immunization of zona pellucida antigen.
目的:评价口服卵透明带DNA避孕疫苗pVAX1-pZP3α壳聚糖纳米微粒诱发的黏膜免疫反应、抗生育作用和对卵巢结构的影响,探讨用黏膜免疫避免卵透明带抗原免疫引起卵巢功能紊乱的可行性。
Objective: To investigate the empty zona pellucida for use in the cryopreservation of human sperm.
目的:探讨空卵透明带作贮存载体冷冻保存少量人类精子。
Zona pellucida protein surrounding to oocyte is important in the proc ess of fertilization.
卵透明带蛋白围绕在卵母细胞外 ,在受精过程中起着重要作用。
Separation, purification and immunological property of chemically deglycosylated porcine zona pellucida antigens DGZPB and DGZPC;
去糖基猪卵透明带抗原DGZPB和DGZPC的分离纯化及免疫特性
Construction and expression of a recombinant adenovirus with LZP3;
草原兔尾鼠卵透明带3基因重组腺病毒载体的构建及表达
Objective:To analyze the epitopes of the recombinant truncated porcine zona pellucida protein 3β(tunc-pZP3β)based on the known antigenic sequence.
目的:分析重组猪卵透明带-3β截短型蛋白(tunc-pZP3β)的结构,以及重组蛋白中产生强免疫原性的氨基酸序列的抗原表位。
Objective: To obtain two different porcine zona pellucida proteins (pZP3β) in amino acid length, pZP3β161 and pZP3β263, and to further study whether the translation initiation region (TIR) effects expression efficiency of exogenous gene in E.
目的:探讨翻译起始区(translation initiation region,TIR)突变对两种氨基酸序列长度不同的猪卵透明带蛋白pZP3β161和pZP3β263在大肠杆菌中表达的影响。
AIM: To improve prokaryotic expression level of Lagurus lagurus zona pellucida 3(LZP3) gene.
目的:探讨提高草原兔尾鼠(Lagurus lagurus)卵透明带3(LZP3)基因在原核细胞中表达的水平。
Mammalian zona pellucida 3(ZP3) plays an important role in the induction of capacitating sperm acrosome reaction.
哺乳动物卵透明带3(Zona pellucida 3,ZP3)在诱导获能精子发生顶体反应中发挥着重要作用,其在大肠杆菌中主要以包涵体形式表达。
MOLECULAR BIOLOGICAL STUDIES ON THE SPERM RECEPTOR OF ZONA PELLUCIDA;
卵透明带精子受体的分子生物学研究
Purification of Recombinant Human Zona-pellucida-3(rhZP3) and Application of rhZP3 in Detection for Antibodies Against Zona Pellucida;
重组人卵透明带蛋白-3（rhZP3）的纯化及其在透明带抗体检测中的应用
Studies of the Biological Activities of the Mutant Recombinant Human Zona Pellucida Protein 3(mrhZP3);
突变重组人卵透明带蛋白3的生物活性研究
The Immunocontraceptive Effect of Zone Pellucida 3 (ZP_3) DNA Vaccines;
卵透明带3（ZP_3）DNA疫苗免疫不育效果的研究
Studies of the Biological Activities of the Recombinant Human Zona Pellucida-3 Protein (rhZP3);
重组人卵透明带蛋白3（rhZP3）的生物活性研究
The Study on Chitosan/zona Pellucida DNA Oral Contraceptive Vaccine;
壳聚糖/卵透明带DNA口服避孕疫苗的研究
Construction and Purification of Recombinant Adenovirus with Porcine Zona Pellucida 3α Gene
猪卵透明带3α重组腺病毒的构建及纯化
Identification and comparison of the variety expression of recombinant human zona pellucida-3
人卵透明带蛋白3差异表达菌的鉴定及比较
Construction integrative lactobacillus casei expressing porcine zona pellucida-3α
构建表达猪卵透明带抗原(pZP3α)的重组乳酸杆菌
Construction of the Human Zona Pellucida Protein 3 Mutant and Its Expression in Pichia Pastoris;
人卵透明带蛋白3突变体的构建及其在毕赤酵母中的表达
The Study on Recombinant Interleukin 5 as Adjuvant of Zona Pellucida DNA Contraceptive Vaccine in Mucosal Immunization;
重组IL-5作为卵透明带DNA避孕疫苗粘膜免疫佐剂的研究
Evaluation of Two Mucosal Immune Routes for Chitosan/zona Pellucida DNA Contraceptive Vaccine;
壳聚糖/卵透明带DNA避孕疫苗两种粘膜免疫途径的评价
Antifertility Effects of Zona Pellucida 3 DNA Vaccine in Lagurus Lagurus;
草原兔尾鼠卵透明带3（ZP_3）DNA疫苗免疫不育的研究
The Secretory Expression of Recombinant Porcine Zona Pellucida Glycoprotein-3α(rpZP3α) in Pichia Pastoris and the Determination of Its Activity;
重组猪卵透明带抗原-3α在毕赤酵母中的分泌表达及活性鉴定
Expression and Purification of Recombinant Human Zona Pellucida 3 Protein in Pichia Pastoris;
重组人卵透明带ZP3蛋白在毕赤酵母中的表达和纯化
The Effects on DNA Vaccine of Murine Zona Pellucida 3 with Interleukin-2;
细胞白介素2影响小鼠卵透明带3（mZP 3）DNA疫苗免疫效果的研究
Cloning, Identification and Analysis of the Full-length cDNA Coding for Lagurus Lagurus Zona Pellucida Glycoprotein ZP3;
草原兔尾鼠卵透明带3 cDNA的克隆、鉴定与分析的研究
Relationship between the Zona Pellucida of Oocyte and Embryo Quality Analyzed by Polscope;
采用偏振光显微镜分析人卵透明带与胚胎发育潜能的关系