Methods Polymerase chain reaction and DNA sequencing were used to detect the mutation hot spot regions of EXT1 and EXT2 gene, while restriction fragment length polymorphism was performed to screen the mutation.
方法:采用聚合酶链式反应结合DNA直接测序法检测EXT1以及EXT2基因的突变热点区域,并利用PCR-RFLP检测技术进行突变筛查。
Relationship between JAK2 V617F point mutation and clinical characteristics of BCR-ABL negative myeloproliferative diseases patients;
JAK2V617F点突变与BCR-ABL阴性骨髓增殖性疾病临床关系研究
Identification of disease-causing point mutations in DMD patients dystrophin gene without large deletions/duplications;
非缺失/重复型Duchenne肌营养不良症患者的致病点突变分析
Study of mitochondrial DNA point mutations at positions 3243, 8993 in hereditary ataxia;
遗传性共济失调线粒体DNA3243、8993点突变的研究
A research on the cusp catastrophe model of rockburst at the working surface of coal mining;
采掘工作面发生冲击地压的尖点突变模型研究
Application of cusp catastrophe model to the groundwater specific vulnerability assessment;
尖点突变模型在地下水特殊脆弱性评价中的应用
Study on ship rolling and capsizing based on the cusp catastrophe
基于尖点突变的船舶横摇倾覆研究
Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase;
CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究
Study on site-directed mutagenesis and kinetics of trehalose synthase from Thermobifida fusca;
Thermobifida fusca海藻糖合成酶的定点突变及其动力学性质研究
The Expression of hBmp-4 in the Pichia Pastoris and Its Site-Directed Mutagenesis;
hBmp-4在甲醇酵母中的表达及其定点突变
Optimization of PCR Amplification Parameters for Mutational Hotspots in the Human Catalase Gene
CAT基因突变热点区域的PCR扩增方法
Study on the Hotspot Mutations of the Wilson Disease Gene in Hunan and Multiplex PCR Reverse Hybridization Technology;
湖南地区肝豆状核变性基因突变热点和多重PCR反向杂交技术研究
The New Technique Platforms for Screening and Detecting the Mutational Hot Spots of Genes Associated with M.tuberculosis Drug Resistance;
耐药结核分枝杆菌基因突变热点筛查与检测技术平台的建立
Extremely mutable sites are called 'hot spots'.
极端可突变的位置称为“热点”。
Research on Function and Oligonucleotide Microarray Detection of Cytochrome P450 Hot Mutation;
药物代谢酶CYP450热点突变检测芯片的研制与应用
The Mutational Hotspots of PIK3CA in Breast Cancer
乳腺癌组织中PIK3CA基因热点突变区域研究
The sudden change in the situation in Afghanistan after the September 11th incident has become a major hot spots in the present world.
“9·11”事件后，阿富汗形势突变，一跃成为目前世界一大热点。
Study on the Hot Spot Mutation of SLC26A4 Gene and LVAS in Sensorineural Hearing Loss with Unknown Reason;
不明原因感音神经性聋SLC26A4基因热点突变与LVAS的研究
Preparation and Application of Genechip for Genotyping the Hot Mutations of Drug Metabolizing Enzyme;
药物代谢酶基因热点突变检测芯片的研制与应用
Oligonucleotide Chip Kit for Cytochrome P450 Hot Mutation in Asian;
CYP450亚洲人群热点突变基因芯片检测试剂盒的研制
Improvement of Cellulase EGV Thermostability by Site-directed Mutagenesis at 49P(del)
49P(del)点突变提高中性纤维素内切酶EGV热稳定性的初步研究
Mutational hotspots of PIK3CA in breast cancer among Chinese women in shanghai
上海地区妇女乳腺癌组织PIK3CA基因热点突变区域研究
Site-directed Mutagenesis and Random Mutagenesis of Penicillium Expansum Lipase;
扩展青霉脂肪酶的定点突变及随机突变
The dots turn red as they register heat.
温度点变红以标记热度．
Analysis on the Abrupt Change Points of Chinese Temperature in the Last 120 Years
近百余年我国气温变化的突变点分析
The fine weather / The heatwave broke at last.
好天气[热天气]终於突然转变了.
The Conversion from the Traditional Conflict Rules to the Modern Conflict Rules;
传统冲突法向现代冲突法的转变及其特点
The Change of the Function and Structure of the Molecular Mutant of Thermostable Alkaline Phosphatse;
耐热碱性磷酸酶分子突变体的结构与功能变化