Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
【Method】 The woodchuck hepatitis virus posttranscriptional regulatory element(WPRE) was amplified by polymerase chain reaction(PCR)from the plasmid containing WPRE.
【方法】将PCR扩增土拨鼠肝炎病毒(Woodchuck Hepatitis Virus,WHV)转录后调控元件(WPRE),克隆于pGM-T载体获得重组质粒pGM-WPRE,利用NotⅠ酶将WPRE亚克隆入pcPPTPRE(+),筛选WPRE正向连接的重组质粒pcPPTWPRE,采用磷酸钙法分别将pcPPTWPRE和pcPPTPRE(+)转染HEK293细胞和DF-1细胞,利用荧光显微镜观察EGFP蛋白的表达,利用流式细胞仪检测转染细胞中EGFP阳性细胞的表达率。
Objective:To investigate the roles of CD4+CD25+regulatory T cells(Treg) in two kinds of murine hapetitis induced by murine hepatitis virus type 3(MHV-3).
方法:通过腹腔注射3型鼠肝炎病毒(MHV-3),在Balb/cJ和A/J两种小鼠上分别建立暴发型肝炎和无症状携带者两类小鼠病毒性肝炎模型,观察病毒感染后两种小鼠肝脏病理变化,检测其血清中的AST、ALT和TBil水平,比较感染后不同时间点脾脏和外周血CD4+CD25+Treg细胞的比例。
Objective:To construct attenuated Salmonella delivering mouse hepatitis virus DNA vaccine and to study its immunogenicity.
目的:构建以减毒沙门氏菌为载体的小鼠肝炎病毒DNA疫苗,研究该疫苗的免疫原性。
Mouse Hepatitis virus(MHV) is a common coronavirus of laboratory mice.
小鼠肝炎病毒(MHV)是一种动物冠状病毒。
Mouse Hepatitis Virus(MHV), a coronavirus of coronavirus genus, has a linear single-stranded RNA genome with positive polarity and causes a variety of diseases, such as enteritis, hepatitis and demyelinating encephalomyelitis in susceptible rodents depending on the virus strain, animal species and immune state of animals, etc.
小鼠肝炎病毒(Mouse Hepatitis Virus, MHV)属于冠状病毒科冠状病毒属(Coronavirus),是一个单股正链RNA病毒,带毒小鼠广泛存在于世界各国的实验小鼠群中。
Changes in Viral Specific IgG and IgM Antibodies Titer after in Fection of Murine Hepatitis Virus in Different Mice Strains and Detection of the Virall;
不同品系小鼠感染小鼠肝炎病毒后IgG、IgM抗体变化及肝脏病毒检测
Method for examination of mouse hepatitis virus in laboratory animal
GB/T14926.22-1994实验动物小鼠肝炎病毒检验方法
Current Status of Research on Mouse Hepatitis Virus (MHV) Spike Protein and It's Receptors
小鼠肝炎病毒S蛋白及其受体的研究现状
Investigation on Mouse Hepatitis Virus Infection of Laboratory Mouse in Guangdong Province
广东省屏障设施小鼠群中小鼠肝炎病毒感染情况
Experimental study on the antivirus effect of Shuanghuanglian and herba houttuyniae on mouse hepatitis virus strain Ⅲ in vitro
双黄连、鱼腥草注射液抗鼠肝炎病毒3型的体外实验研究
Establishment of HCV Infection Model Using a Mouse Hepatoma Cell Line;
丙型肝炎病毒感染的小鼠肝癌细胞模型研究
Development of the mouse model for acute hepatitis B virus infection
急性乙型肝炎病毒感染小鼠模型的建立
A mouse model of acute hepatitis B virus infection constructed by hydrodynamic injection
水动力注射法构建乙型肝炎病毒感染小鼠模型
Antiviral Effects of Small Interfering RNA in Hepatitis B Virus Infected Mice;
小干扰RNA在乙型肝炎病毒感染小鼠中的抗病毒作用
Study on Protein Expression Pattern in Transgenic Mice Harbouring HBV Genomic DNA;
乙型肝炎病毒全基因组转基因小鼠病毒蛋白表达模式的研究
Entecavir inhibits hepatitis B virus(HBV) replication in HBV replication mice
恩替卡韦抑制乙型肝炎病毒复制型小鼠体内病毒的复制
HBV Infection and Hepatitis B Virus Associated Glomerulonephritis;
乙肝病毒感染与乙肝病毒相关性肾炎
Microarray Analysis of Gene Expression in Liver Tissue from Balb/cJ Mice with MHV-3-induced Fulminant Hepatitis
小鼠暴发型病毒性肝炎肝组织基因表达谱的分析
non A non B hepatitis virus
非甲非乙型肝炎病毒
hepatitis caused by a virus.
由于病毒引起的肝炎。
hepatitis c/co-infection with HIV
艾滋病毒和肝炎病毒重迭感染
SEN virus, a recently discovered hepatitis viruses?
SEN病毒:一种新近发现的肝炎病毒?
Studies on Establishment and Characteristics of Hepatitis G Virus Transgenic Mouse Models
庚型肝炎病毒转基因小鼠模型的建立及其特性研究