Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
【Method】 The woodchuck hepatitis virus posttranscriptional regulatory element(WPRE) was amplified by polymerase chain reaction(PCR)from the plasmid containing WPRE.
【方法】将PCR扩增土拨鼠肝炎病毒(Woodchuck Hepatitis Virus,WHV)转录后调控元件(WPRE),克隆于pGM-T载体获得重组质粒pGM-WPRE,利用NotⅠ酶将WPRE亚克隆入pcPPTPRE(+),筛选WPRE正向连接的重组质粒pcPPTWPRE,采用磷酸钙法分别将pcPPTWPRE和pcPPTPRE(+)转染HEK293细胞和DF-1细胞,利用荧光显微镜观察EGFP蛋白的表达,利用流式细胞仪检测转染细胞中EGFP阳性细胞的表达率。
Gene synthesis and construction of prokaryotic expression vector of hepatitis B core antigen
乙型肝炎核心抗原基因的合成及其原核表达载体的构建
Study on the Transactivating Function of HBcAg Antigen;
乙型肝炎病毒核心抗原反式激活作用的研究
Construction of HCV Core and Envelope 2 Fusion DNA Vaccine and Its Immunogenicity in Mice;
丙型肝炎核心——包膜E2抗原融合基因核酸疫苗的构建及免疫应答
Expression of the hepatitis Delta antigen in prokaryotic cell and evaluation of its application as an EIA diagnostic reagent
丁型肝炎病毒抗原的原核表达及抗原性分析
Experimental Study on Membrane Expression of Hepatitis B Core Antigen in HepG2 Cells;
乙型肝炎病毒核心抗原在HepG2细胞膜表达的实验研究
The value of HCVcAg detection by a sandwich-ELISA system in clinical diagnosis of HCV infection
丙型肝炎病毒核心抗原检测在临床诊断HCV感染的价值
SYNTHESIS OF GENE ENCODING HEPATITIS B CORE BY OVERLAPPING PCR AND ITS EXPRESSION IN ESCHERICHIA COLI
重叠PCR合成乙型肝炎病毒核心抗原基因及其在大肠杆菌中表达
Construction of duck hepatitis B virus core antigen DNA vaccine and induced humoral immune response
鸭乙型肝炎病毒核心抗原DNA疫苗的构建及其诱导的体液免疫应答
The Qualitative and Quantitative Detection of HCV Core Antigen in the Serum of the Patients with Chronic Hepatitis C by Homemade Enzyme Linked Immunoadsorbent Assay;
慢性丙型肝炎患者血清丙型肝炎病毒核心抗原定性及定量检测的临床意义
Cloning, Expression of a Gene Fragment Encoding HCV Core Antigen and Purification, Antigenicity Analysis of the Recombinant Protein;
丙型肝炎病毒核心抗原基因片段的克隆、表达及蛋白的纯化和抗原性分析
Evaluation of 5 Kinds of China-made Enzyme Immuoassay Kits for Antibody to Hepatitis B Virus Core Antigen Detection
五种国产酶免疫测定法抗乙型肝炎病毒核心抗原抗体检测试剂的评价
The hepatitis C virus immune body double antigen contains filling the law clinical research
丙型肝炎病毒抗体双抗原夹心法的临床研究
Studies on Expression of Fusion Gene HAV Epitopes Antigen with HBV Core Antigen;
甲肝病毒表位抗原与乙肝病毒核心抗原融合基因表达的研究
Detection of hepatitis B virus DNA in blood donors with anti-HBc positive and analysis of S gene variation
乙型肝炎核心抗体阳性献血者乙肝病毒DNA检测及基因变异分析
epidemic hepatitis associated antigen (EHAA)
传染性肝炎相关抗原
HIGH- LEVEL EXPRESSION AND SECRETION OF CHINESE HENAN HEPATITIS DELTA ANTIGEN IN PROKARYOTIC CELLS
我国河南株丁型肝炎病毒抗原在原核细胞中的高效表达及分泌
tigen-antibodycomplex nephritis
肝炎抗原抗体复合物性肾炎
Clinical Significance of Dynamic Evaluation of Combined Detection of Hepatitis B Virus Pre S1 Antigen and Core Antigen
动态评价联合检测乙肝病毒前S1抗原与核心抗原的临床意义