Conclusion Pseudovirus vector of human papilloma virus type 16 could transfect and kill HepG2 cells,which might provide a new method for liver cancer therap.
目的构建基于人乳头状瘤病毒(HPV)16型的伪病毒载体,并检测其对肝癌细胞系HepG2细胞的杀伤活性。
Pseudorabies virus(PRV),a member of alphaherpesvirus,is a novel viral vector to develop bivalent or multivalent genetic engineering vaccines.
以伪狂犬病毒基因缺失标志疫苗株TK-/gG-/LacZ+为亲本株,通过同源重组,构建了表达猪繁殖与呼吸综合征病毒(PRRSV)主要免疫原性蛋白GP5的重组伪狂犬病毒TK-/gG-/GP5+。
5kb antisense RNA fragment was designed targeting the 5’ non-coding region (NCR), translation initiation site and potential transcriptional active region of the sole immediate early gene(IE180) of Pseudorabies virus(PRV).
伪狂犬病毒(Pseudorabies virus,PRV)属疱疹病毒科α 疱疹病毒亚科,基因组为线状双链 DNA,长约150kb,至少可编码 70~100 种蛋白质。
Construction of the Recombinant Adenovirus Expressing gC Glycoprotein Gene for Developing Vaccine against Pseudorabies virus;
表达伪狂犬病毒gC糖蛋白基因重组腺病毒的构建及其免疫效果
Cloning, sequence analysis of the gG gene of Pseudorabies virus SH strain;
伪狂犬病毒上海株糖蛋白G (gG)基因的克隆及序列分析
Construction of pseudorabies virus genomic library and analysis of physical mapping;
伪狂犬病毒闽A株基因文库的构建及物理图谱的分析
The expression of the immunogenic glycoprotein D(gD) of pseudorabies virus (PRV) in transgenic tobacco was reported.
将猪伪狂犬病毒 (pseudorabiesvirus ,PRV)最主要的保护性抗原基因gD完整编码区亚克隆到修饰的植物双元表达载体pBI 35SL中 ,使其置于强启动子CaMV 35S doubleenhancer TEV 5′UTR下游 ,构建的转基因植物双元表达质粒经农杆菌介导转化烟草 。
0 kb DNA fragment containing the modified enhanced green fluorescent protein CDNAM1 (EGFP S147/P) andSV40 poly(A) signal sequence was amplified and cloned in frame behind the first eight codons of the nonessential glycoprotein G (gG) of pseudorabies virus (PRV) to yield a transfer plasmid.
将绿色荧光蛋白突变体M 1(EGFPS14 7/P)基因融合到伪狂犬病毒 (PRV)非必需糖蛋白 gG的第 8个氨基酸下游 ,通过同源重组、空斑纯化和PCR筛选获得能表达M 1并导致gG基因部分缺失的重组病毒 gG-/M1+ 。
CONSTRUCTION OF PRV EXPRESSING VECTOR AND IDENTIFICATION WITH E.coli β LacZ;
伪狂犬病毒表达载体的构建
The product of expression was specific to antisera against PRV antigen.
利用PCR技术从猪伪狂犬病毒基因组中克隆gE抗原表位基因,并将其插入到载体pET-22b(+)中,构建成原核表达质粒pET-22b(+)-gE,使其在E。
Pseudorabies virus (PRV) is the causative agent of Pseudorabies (Aujeszky's disease)of many domestic and wild animals.
伪狂犬病毒(Pseudorabies virus，PRV)可引起多种家畜和野生动物的伪狂犬病，尤其是猪伪狂犬病，给世界养猪业造成了巨大的经济损失。
C3d-M28 enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus
C3d-M28增强伪狂犬病毒gC基因体液免疫
Detection of PRV、PPV and PCV2 Infection Using PCR;
应用多重PCR方法检测猪伪狂犬病毒细小病毒和圆环病毒2型
Tissue Distribution of the Latent PRV in the Pigs;
伪狂犬病毒在潜伏感染猪体内的组织分布
Spatio-Temporal Gene Expression during Lantency of Pseudorabies Virus;
伪狂犬病毒基因组在潜伏感染中的时空表达
Study on Complement C3d Enhancement of Pseudorabies Virus gC Gene Immunogenicity;
补体C3d增强伪狂犬病毒gC基因免疫原性的研究
Construction and Expression of Recombinant PRV PK Gene Deleted Transfer Vector;
伪狂犬病毒PK基因缺失转移载体的构建和表达
Cloning, Expression and Detection of the gD Gene of Porcine Pseudorabies Virus Ja Strain;
猪伪狂犬病毒冀A株gD基因的克隆、表达及检测
Isolation and Identification of Porcine Pseudorabies Virus and Establishment of Detection Methods;
猪伪狂犬病毒的分离鉴定及检测方法的建立
Studies on a Silver-Enhanced Colloidal Gold Assay for the Detection of Pseudorabies Virus Antigen;
银加强胶体金标记技术检测伪狂犬病毒的研究
Study on the Recombinant Virus Live Vaccine of Pseudorables Virus and Hog Cholera Virus;
猪瘟伪狂犬病重组病毒活疫苗的研究
Diagnosis Establishment of PCR Detection for Pseudorabies Virus;
猪伪狂犬病病毒PCR诊断方法的建立
The Eukaryotic Expression of gK Gene of Pseudorabies Virus Ea Strain
伪狂犬病病毒Ea株gK基因的真核表达
Construction of Recombinant Pseudorabies Virus Expressing the Rabies Virus Glycoprotein and the Transient Expression;
狂犬病病毒糖蛋白重组伪狂犬病病毒载体构建及瞬时表达
Construction of a recombinant pseudorabies virus(Bartha-K61) reversely-expressing glycoprotein of rabies virus
反向表达狂犬病病毒糖蛋白的重组伪狂犬病病毒Bartha-K61株的构建
Study on Functions of PRV Major Virulent Genes;
伪狂犬病病毒主要毒力基因功能的研究
Expression of Pseudorabies Virus Glyprotein E in E Coli;
伪狂犬病病毒gE基因在大肠杆菌中的表达
Bioinformatics Analysis of Pseudorabies Virus gD Gene;
伪狂犬病病毒gD基因的生物信息学分析
Application of Cre/loxP System in Pseudorabies Recombinant Virus;
Cre/loxP系统在重组伪狂犬病病毒中的应用