Objective:To evaluate the difference between 5-diphenyl tetrazolium bromide(MTT)and cytopathic effect(CPE)in screening the antiviral medicine.
目的:比较四甲基偶氮唑盐(MTT)法与细胞病变效应(CPE)在筛选抗病毒药物中的异同。
We observed the cytotoxicity of Qingfei quyu decoction on A549 cells by using MTT method;after that,observed the protective effect for the cytopathic effect of A549 cells by Qingfei quyu decoction below media
实验部分:清肺祛瘀汤抗RSV的体外实验,观察清肺祛瘀汤对A549细胞毒性,用MTT法测定药物的细胞毒性;再以半数毒性浓度下的清肺祛瘀汤,观察其对A549的细胞病变效应(CPE)的保护作用,以了解药物的体外抗病毒作用。
By using cytopathic effect (CPE) as the index, the antivirus effects in vitro and cytotoxicity of five Chinese drug preparations were investigated.
方法建立流感病毒感染MDCK细胞、柯萨奇病毒和呼吸道合胞病毒感染VeroE6细胞的模型,以细胞病变效应(CPE)为观察指标,了解不同浓度5种中药制剂体外抗病毒效果及细胞毒性。
Methods The cell killing effect was observed by CPE(cytopathic effect) assay;the cell growth inhibition and virus propagation efficiency was measured by MTT assay and plaque formation assay,respectively.
方法:通过细胞病变效应(CPE)观察d l1520对肝癌细胞株的杀伤作用;MTT法测定对肝癌细胞生长抑制作用,并通过空斑法测定病毒增殖,探讨其杀伤机理。
The morphological observation of Guizhou NDV isolate and cytopathogenic effect of infected cells;
贵州新城疫分离物的形态学观察与细胞病变效应
The infected chorioallantoic membrane became thicken,congestive and pox formation,the affected monolayer of BHK(21) manifested cytopathogenic effect,and all these phenomena could be inhibited by reference serum of goat pox.
通过电镜观察在痘疹材料中发现大量典型的痘病毒粒子;感染鸡胚后绒毛尿囊膜增厚、充血和出现痘斑;接种BHK21单层细胞引起明显的细胞病变效应,而且这种现象能够被山羊痘参考血清所抑制。
Methods CPE observation and plaque reduction assay were used to test the anti-HSV-1 effect of Ilex Kudmcha C.
方法采用细胞病变效应法(Cytopatho-genic effect,CPE)和空斑减数实验(Plaque reduction assay)测定不同苦丁茶提取物抗HSV-1活性,计算药物对病变的抑制率和半数抑制浓度(50%inhibiting concentration,IC50),并从药物对细胞的保护,对病毒增殖的影响及对病毒感染细胞的综合作用3个方面初步探索苦丁茶提取物抗HSV-1病毒活性的机理。
No cytopathic effect (CPE) can be observed for CSFV when cultured in vitro on host cells though serious damages occur in many tissues and organs for in vivo infection in pigs.
猪瘟病毒感染造成机体多种组织损伤，但在离体培养细胞中通常不产生致细胞病变效应(cytopathic effect, CPE)，对猪瘟病毒致病机制的研究，因为缺少合适的细胞研究模型而进展缓慢。
Objective To observe Cytopathic Effect of wild-type mump viruses in Vero/SLAM cell and identify genotype in a mump outbreak in 2008.
目的对吉林省2008年一起流行性腮腺炎(腮腺炎)爆发疫情进行病原学监测,观察是否出现典型的致细胞病变效应(Cytopathic Effect,CPE),并进行病毒核酸和基因型鉴定。
In this article,research advances in BVDV were described systematically,including the structure and function of BVDV virion,replication cycle and polyprotein cleavage,cytopathic effect and immune escape mechanism.
论文从牛病毒性腹泻病毒的病毒粒子结构组成及功能、病毒复制周期及蛋白裂解过程、细胞损伤及免疫逃逸机制三个方面阐述了近几年牛病毒性腹泻病毒分子生物学研究的进展,重点讨论了病毒复制周期、多聚蛋白裂解、致细胞病变效应和免疫逃逸机制。