For better understanding the molecular characteristics of the genes and proteins of a novel adult diarrhea rotavirus strain J19(NADRV-J19),the complete genome set of NADRV-J19 was amplified by an improved single primer sequence-independent amplification method,cloned into pMD18-T vector,and sequenced.
为了进一步了解新成人腹泻轮状病毒J19株的基因和蛋白特征,利用一种改良的非依赖核酸序列的单引物扩增方法扩增J19株的11个基因,克隆到pMD18-T载体中并进行测序。
Genes of a novel adult diarrhea rotavirus(NADRV) strain J19 were amplified by an improved single primer sequence-independent amplification method,cloned into pMD18-T vector,sequenced and analyzed.
利用一种改良的非依赖核酸序列的单引物扩增方法,从新成人腹泻轮状病毒J19株的核酸中扩增基因,克隆至pMD18-T中并进行测序和基因序列分析。
Clinical observation of therapeutic effect of zinc gluconate on infantile rotavirus diarrhea;
葡萄糖酸锌治疗小儿轮状病毒腹泻332例疗效观察
Research Progress of the Bovine Viral Diarrhea Virus-Mucosal Disease Prevalence in Current and Control
牛病毒性腹泻-粘膜病流行现状及防制研究进展
Bovine viral diarrhea virus(BVDV) leads to BVD-MD,which prevails worldwide.
牛病毒性腹泻病毒(BVDV)引起牛病毒性腹泻-黏膜病,广泛分布于世界各地。
Bovine viral diarrhea virus (BVDV) is the member of pestivirus genus in the Flaviridae family, the virion is globular and enveloped with genome is single-strained positive-strain RNA approximately 12-13kb in length.
牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)是黄病毒科、瘟病毒属成员,病毒粒子呈球形,有囊膜,核酸为长约12～13kb的单股正链RNA,根据能否使培养细胞产生细胞病变将其分为两种生物型:产细胞病变型和非细胞病变型。
bovine viral diarrhea virus
牛病毒性腹泻病毒[归于黄病毒科瘟病毒属]
Isolation and Identification for Genotype 2 BVDV Isolates in China;
基因2型牛病毒性腹泻病毒的分离、鉴定
Diagnosis of BVDV and Dividing Type Studying Using RT-PCR;
牛病毒性腹泻病毒RT-PCR检测及分型研究
The development of a hybridoma cell strain secreting monoclonal antibody against BVDV1 and BVDV2
抗牛病毒性腹泻病毒单克隆抗体的研制
RT-PCR amplification of bovine viral diarrhea virus E_0 gene from sika deer
鹿源牛病毒性腹泻病毒E_0基因的RT-PCR扩增
Research Progress of the Bovine Viral Diarrhea Virus-Mucosal Disease Prevalence in Current and Control
牛病毒性腹泻-粘膜病流行现状及防制研究进展
Xinjiang Northern Border Partial Intensification Cattle Farm Bovine Viral Diarrhea Virus Molecular Epidemiology Investigation;
新疆北疆部分集约化奶牛场牛病毒性腹泻病毒分子流行病学调查
Serologic investigation of bovine viral diarrhea virus infection on some dairy cow farms in Heilongjiang Province
黑龙江省部分奶牛场牛病毒性腹泻病毒感染的血清学调查
Research of Diagnosis and Prevention Technology Against Diarrhea of Yak on MAQU Country;
玛曲县牦牛病毒性腹泻的诊断与防治技术研究
The Investigation on Diagnosis and Immune Technology of Viral Diarrhea;
牛病毒性腹泻诊断及免疫防制技术的研究
Comparison on Culturing the BDV/MD Virus in Different Cells
牛病毒性腹泻/粘膜病病毒在不同细胞上生长增殖的比较
Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;
反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
Establishment and evaluation of real-time PCR for detection of bovine viral diarrhea virus
牛病毒性腹泻病病毒荧光定量PCR检测体系的建立与评价
Epidemiology Investigation of Bovine Viral Diarrhea Virus in Shihezi,Xinjiang
新疆石河子地区牛病毒性腹泻病毒的分子流行病学调查
Expressing of bovine viral diarrhea-mucosal disease virus Changchun184 strain of E_2 gene in BCG
牛病毒性腹泻-黏膜病病毒Changchun184株E_2基因在卡介苗中的表达
Expression of the p80 Fragments of BVDV and Antigenicity Analysis;
牛病毒性腹泻病毒p80基因的分段表达及其抗原性分析
Preparation of monoclonal antibodies against bovine viral diarrhea virus and evaluation of their characteristics
抗牛病毒性腹泻病毒单克隆抗体的制备及其特性研究
Monitoring of Prevalence Status of BDV MD in Beef Cattle Herds in China
我国规模化肉牛场牛病毒性腹泻-粘膜病流行状况监测