WT5BZ]In order to obtain complete sequence and catalytic core of human tyrosine hydroxylase(TH) cDNA, the full length of cDNA from human TH was used as template and the specific oligonucleotide primers were designed for PCR.
设计合成含适当酶切位点的PCR引物 ,以人的全长THcDNA为模板 ,进行PCR扩增反应 ,得到人酪氨酸羟化酶 (tyrosinehydroxylase,TH)全长及催化核心序列 ,将其分别插入谷胱甘肽巯基转移酶 (GST)融合表达载体pGEX 4T 1 ,转化入大肠杆菌BL2 1 ,以IPTG诱导表达 ,SDS PAGE、westernblotting分析表达产物。
A steady numerical simulation of the effect of nano-particles on nucleation sites in nucle- ate pool boiling heat transfer was conducted based on the experimental boiling curves.
根据实验所得沸腾曲线,对纳米颗粒悬浮液进行稳态数值模拟,计算了不同过热度下活化核心的密度。
Study on How to Built the Core Economy Growth Pole of the Chongqing New North Zone
关于打造重庆北部新区核心增长极的研究
The “Core Effect” in the Development of Small Cities in the Western Area of China;
西部地区小城市发展的“增长极核效应”
The western development requires the prior development in important central cities, which can form growth pole and give an impetus to western region.
西部大开发必然要求优先发展西部重点中心城市，以形成增长极核，发挥辐射带动作用。
Research on Key Factors, Structures and Policies for Economic Growth;
经济增长核心因子、结构与政策研究
Building Technological Core Competence: North China Pharmaceutical Corporation and Changhong as Cases;
企业技术核心能力增长:以华北制药、长虹为例
Building and Developing CBD;
中央商务区:西部中心城市商贸发展增长极
Raise Medium and Small Scale Enterprise Competitiveness,Promote Jilin Province Economic Development;
提升中小企业核心竞争力 促进吉林省经济增长
Institutional innovation: the core for constructing long-term efficient mechanism for peasants income increase;
制度创新是构建农民增收长效机制的核心
ENERGY SAVING AND EMISSION REDUCTION TO BECOME CORE IMPETUS TO THE ECONOMIC GROWTH
节能减排将成为引领经济增长的核心动力
Discussion on the Strategic Development of Qingdao City-From the Point of View of Polar Nucleus Theory of Economic Growth and the Theory of Scale Merit;
青岛城市发展战略探讨——从经济增长极核理论与规模效益理论角度
The Causing Mechanism for Growth Pole and the Relationship between Growth Pole and the Periphery;
增长极形成机制及增长极与外围区的关系
THEORY OF ECONOMIC GROWTH POINTS AND CHOICE OF INNER MONGOLIAN ECONOMIC GROWTH POINTS;
经济增长极理论与内蒙古经济增长极的选择
Cultivate economic growth pole of western region by referring to growth pole patterns of the three economic regions;
借鉴三大区域增长极模式培育西部经济增长极
Even more reassuring are said to be the positive signs of growth in the American economy.
更使人宽心的，据说是美国经济中积极的增长信号。
Base on this mechanism, it analyzes the process and mechanism of the technological core competence growth in Zhongxing Telecom.
然后,以这个机制为基础,重点剖析中兴通讯技术核心能力增长过程与增长机制。
Analysis on Technological Core Competence Growth in Telecom Manufacturer: A Case Study on Zhongxing Telecom;
通讯企业技术核心能力增长分析——以中兴通讯为例
The Increasing Tendency of Document Information Amount of Digital Libraries in China and Evaluation on Core Authors
中国数字图书馆文献信息量的增长态势及其核心著者测评
Dual Growth Poles and the Orderly Development of Changjiang River Ares
双重增长极与长江流域有序发展研究