According to the cDNA sequence of anti neuroexcitation peptide of scorpion Buthus martensii Karsch, the putative mature anti neuroexcitation peptide (ANEP) encoding DNA fragment was obtained by PCR method, and then was cloned into expression plasmid pET28a, fused with His tag at its 3 end.
本文报道了一种新发现的抗神经兴奋肽 (ａｎｔｉ ｎｅｕｒｏｅｘｃｉｔａｔｉｏｎｐｅｐｔｉｄｅ ,ＡＮＥＰ ,Ｇｅｎｂａｎｋ号 :ＡＦ1 2 2 0 0 3)在大肠杆菌中的表达和纯化及其在小鼠试验模型。