Construction and identification of a specific expression vector for SV40 large T antigen in gastric parietal cell;
SV40T抗原胃壁细胞特异性表达载体的构建与鉴定
Expression and sgnificance of T antigen in gastric carcinoma patients;
T抗原在胃癌组织中的表达及临床意义
OBJECTIVE To design and construct eukaryocyte expression vector of SV40 virus large T antigen and induce its targeted expression in eukaryocyte.
目的设计、构建SV40病毒T抗原真核表达载体,并导入真核细胞进行表达鉴定。
Methods We investigated the presence of SV40 T antigen genome in 195 blood specimens from wild monkeys(71 from Nin Lang county,60 from Jin Dong county in Yunnan province) and 64 from caged monkeys by means of the polymerae chain reaction(PCR) followed by sequencing.
目的建立猴外周血单核细胞SV40 DNA的PCR检测方法,对猕猴SV40 T抗原基因进行检测。