The cDNAs of SIV 8 gene segments were synthesized by RT-PCR and cloned into the RNA polymerase I/II bidirection expression vector PHW2000 independently, resulting in 8 recombinant plasmids.
将猪流感病毒8个基因节段经RT-PCR合成cDNA后,分别克隆到RNA聚合酶I/II双向表达载体PHW2000中,构建成8个重组质粒。
The cDNAs of SIV 8 gene segments were synthesized by RT-PCR and cloned into the RNA polymerase Ⅰ/Ⅱ bidirection expression vector PHW2000 independently,resulting in 8 recombinant plasmids.
方法:将猪流感病毒8个基因节段经 RT-PCR 合成 cDNA 后,分别克隆到 RNA 聚合酶Ⅰ/Ⅱ双向表达载体 PHW2000中,构建成8 个重组质粒。
A method for real-time editing and rendering of translucent materials with homogeneous diffuse bidirectional surface scattering reflectance distribution function(BSSRDF)is proposed.
半透明物体透明效果的真实感绘制是近年来研究的热点,提出一种针对半透明物体漫散射效果的实时真实感绘制与材质动态编辑方法——基于双向表面散射反射率函数(BSSRDF)的Dipole近似。