In this study, we investigated the assembly of Asia 1 foot-and-mouth disease virus empty capsid particles in insect cells using baculovirus expression system based on this new incurred Asia 1 FMDV, and with the hope to achieve a safe, high potency subunit vaccine based on empty capsid particles for Asia 1 FMDV.
采用了三种不同的策略来研究Asia 1型FMDV空衣壳的组装:一是将P12A和3C基因序列分别插入分泌型杆状病毒表达载体pMel-Bac B中,探讨目的基因的分泌表达;二是将空衣壳组装必需的3C基因和P12A基因插入带双启动子的表达载体pFastBac? Dual中,两种基因分别置于不同的启动子控制之下,同时表达P12A和3C蛋白,在昆虫细胞内裂解组装空衣壳粒子;三是通过耐酸性改造,研究提高空衣壳酸稳定性的条件;当pH值低于7时,FMDV二十面体对称的衣壳结构就会裂解成12S的五聚体,而昆虫细胞培养基的正常pH值在6。
This paper first put forward the concept and idea of reasonable and durable structure construction.
混凝土梁式桥结构构造合理与否直接影响结构的受力性能和耐久性能,目前国内针对合理耐用结构构造的研究非常欠缺,只是在工程实践基础上总结了一些构造措施,没有针对合理结构构造进行理论分析。
Synthesis of Foot-and-Mouth Disease Virus Empty Capsids in Insect Cells Through Acid-Resistant Modification
通过耐酸性改造在昆虫细胞中组装FMDV空衣壳结构
Preparation of waterproof acrylate emulsion modified by dimer acid
二聚酸改性丙烯酸酯耐水乳液的合成
Effect of Additives on Properties of Water Glass Acid-Resistant Mortar;
外掺料对水玻璃耐酸砂浆的改性作用
Breeding Actinobacillus Succinogenes with Acid-tolerance by Genome Shuffling
基因组改组技术选育耐酸性琥珀酸放线杆菌
Effect of acid fluid on carbonate reservoir reconstruction
酸性流体对碳酸盐岩储层的改造作用
Aluminum Pigments Encapsulated with Nano-SiO_2 Coatings and Their Acid-resistance
纳米SiO_2包覆改性薄片铝粉颜料及其耐酸性研究
Study on Manufacturing Process and Mechanical Property of Modified High Manganese Wear-resistant Steel Ring-hammer
改性高锰耐磨钢环锤制造工艺及性能研究
Study on improving the corrosion resistant property of Portland limestone cement
改善石灰石硅酸盐水泥耐腐蚀性能的研究
Characterization of Acid Tolerance of Genome-shuffled Strain of Lactobacillus casei rhamnosus
干酪乳杆菌基因组改组菌株耐酸性表征
Preparation of the Acrylate Polymers Used for Modification of POM and the Ageing-resistance of the Modified POM;
聚甲醛改性用烯酸酯聚合物的制备及改性聚甲醛的耐老化性能
Synthesis of Phosphate Modified Hydroxyl Acrylic Emulsion and Its Anti-Corrosion Properties
磷酸酯改性羟基丙烯酸乳液合成与耐腐蚀性研究
Study on Surface Durability Improvement Measures of Pavement Concrete
路面混凝土表面构造耐久性改善技术研究
Technology of Improving Surface Corrosion Resistance of Cast Aluminum Alloy
改善铸造铝合金表面耐腐蚀性能的技术
Ethanol tolerance in yeast:molecular mechanisms and genetic engineering
酿酒酵母乙醇耐性的分子机制及基因工程改造
Water Resistance and Mechanical Properties of Acrylate Copolymer Modified by Waterborne Epoxy Resin
水性环氧树脂改性丙烯酸共聚物的耐水性和力学性能
In retrofit, measures of not varying the main body and changing over to use membrane economizer for enhancing wear-resistant capability of the heating surface have been taken.
改造采取主体不变，改用膜式省煤器的措施来提高受热面的耐磨性。
The Experimental Study on PMMA Artificial Tooth to Improve the Hardness and Antiwear with Nanotechnology;
纳米技术提高聚甲基丙烯酸甲酯人造牙硬度及耐磨性的实验研究
Sieving of Lactic-Acid Bacteria Resisting Salt in Soy Fermentation and Its Appraisement of Molecular Biology;
酱油酿造中耐盐性乳酸菌的筛选及分子生物学鉴定