The plasmid p389ARG7 is transformed into arginine dependent and cell wall-less strains CC-1618 (arg7cw15mt-) and CC-1617 (arg7cw15mt-) of Chlamydomonas reinhardtii by using glass bead method respectively, and some insertion mutated transformants with various background have been obtained.
用微玻璃珠研磨法将 p ARG7质粒转入到莱茵衣藻精氨酸依赖型、缺壁突变株品系 CC-1 61 8( arg7cw1 5 mt-)和 CC-1 61 7( arg7cw1 5 mt-)中 ,得到了一些不同的插入型突变转化子 。
The plasmid p387NIT1 was transformed into nitratase deficient and cell wall-less strain CC-2677(cw15nit1-305mt-) of Chlamydomonas reinhardtii by using glass bead method, and some insertion mutatedtransformants with various background have been obtained.
用微玻璃珠研磨法将p387NIT1质粒转入到莱茵衣藻硝酸还原酶缺陷型及缺壁突变株品系CC-2677(cw15nit1-305mt-)中,得到了一些不同的插入型实变转化子,在本实验室条件下CC-2677突变株品系的转化率最高时达到了88个/μgDNA,经多次转化共获得各种转化子1800多个,对它们进行荧光检测后检测出缺乏叶绿素b的一个突变子NIT17-1,并进一步用高效液相色谱法对其光合色素含量进行检测,结果证实该突变体的确丧失了叶绿素b合成能力,该突变株将可作为CBN1基因克隆及其定位的有利工具。
Chinese scholars have been doing Pearl Buck studies for more than 70 years and have made remarkable achievements especially since the New Phase in the 20th Century,which owes a great deal to the use of multiple research methods and perspectives besides the influence of the age of globalization and the new international cultural context.
中国赛珍珠研究已经走过了70余年的历程,特别是新时期以后,这一研究取得了丰硕的成果,而这些成果的取得除了由于赛珍珠及其创作在全球化时代越发显现出其现实意义外,还得益于多样的研究方法的运用。
Guo Yingjian thinks that Pearl Buck studies in today’s China are lacking in the close readingand analysis of numbers of her works,hence an intentional or unintentional neglect of her literary value.
郭英剑认为当今中国大陆赛珍珠研究存在的主要问题是缺乏对赛珍珠众多作品的详细解读和分析,因此赛珍珠的文学价值被有意无意地忽略或是贬低了,因此,应该加强对赛珍珠文学作品的研究。
Pearl S. Buck Studies in the Context of Globalization;
全球化语境中的赛珍珠研究
Relatively speaking, recent Pearl S.
近年中国赛珍珠研究取得了较为丰硕的成果,但相对于赛珍珠作品的数量和质量而言,已有的研究还很不够。
Methods: SD rats were randomly pided into three groups and supplied with nanometer and micrometer pearl powder at different dosages.
方法:70g左右的SD大鼠100只,以低钙基础饲料饲喂2w后分别添加5%、10%的纳米珍珠粉和微米珍珠粉,另10只大鼠为实验本底组。
Pearl S. Buck Studies: the Research Methods and Perspectives-A Discussion Starting from the Present Studies;
我们今天怎样研究赛珍珠——从中国赛珍珠研究现状谈起
Important Issues for Exploration in Pearl S. Buck Studies;
关于赛珍珠研究的几个有待深入的问题
Pearl S. Buck′s Drawing From Dickens′ Novel Writing--Also A Discussion on Western Culture in the Study of Pearl S. Buck
赛珍珠对狄更斯小说创作的借鉴——兼论赛珍珠研究中的西方文化因素问题
If you want to understand today, you have to search yesterday.-- Pearl Buck
想要懂得今天，就必须研究昨天。——赛珍珠
A Study of the Cultural Hermeneutics in Pearl S. Buck s Translation of All Men Are Brothers;
赛珍珠英译《水浒传》的文化阐释研究
A Comparison between Pearl S.Bucks Novels and 1930s Chinese Local Novels;
赛珍珠小说与30年代中国乡土小说比较研究
An Assessment of Pearl S. Buck Symposium, Zhenjiang, China, 2002;
2002中国镇江赛珍珠学术研讨会综述
If you want to understand today, you have to search yesterday.--Pearl Buck, American female writer
想要懂得今天,就必须研究昨天。美国女作家赛珍珠
Elimination and Rectification--Reflection on the contemporary studies of Pearl Buck and her 《The Good Earth》;
历史限禁与文学祛蔽——对赛珍珠及其《大地》研究的当下反思
Pearl S.Buck s Heritage Increases with Time--An Overview of Pearl S.Buck Symposium,Zhenjiang,China;
珍珠遗产 历久弥新——2005中国镇江赛珍珠学术研讨会综述
In present paper, the structure of nacre and coating pearl powder on titanium substrate by Electrophoretic Deposition(EPD) were investigated.
文中首先研究了片状珍珠粉的制备工艺,分析了贝壳珍珠层与珍珠粉的结构。
"The Earth" Lifts up the "Pearl," the "Pearl" Illumines "the Earth"--Tentatively on Pearl Buck and her Representative Work The Good Earth;
“大地”托起“珍珠” “珍珠”辉映“大地”——刍议赛珍珠及其代表作《大地》
Shigeru Akamatsu is the senior manager at Mikimoto's Pearl Research Lab.
SHIGERU AKAMATSU是MIKIMOTO珍珠研究实验室的高级主管。
Genetic Diversity and Protection Strategy of Buxus.sinica var.parvifolia;
珍珠黄杨遗传多样性研究及保护对策
Study on Content of Zn and Se in Wild Lysimachia Clethroides about Chizhou;
池州野生珍珠菜中Zn、Se含量的研究
Preparation and Study of the Nano-Nacre Artificial Bone
纳米珍珠层人工骨的制备及实验研究
Study on Nuclear Ribosomal DNA ITS Region Sequence of Buxus sinica var. parvifolia
珍珠黄杨nrDNA的ITS序列片段研究
The Separation Appraisal and Drugs Susceptibility Test of Escherichia Coli in Guinea
珍珠鸡大肠杆菌敏感性药物筛选研究