Methods: Distribution of negative charges in different phases of PHN on glomerular basement membrane were measured with label method with polyethyleneimine (PEI) in vitro as a cation tracer.
方法 :以阳离子多聚体 (PEI)为阳离子示踪剂 ,采用体外标记法检测PHN不同时相肾小球基膜阴电荷分布情况。